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Molecular Biology of B Cells is a comprehensive reference to how B cells are generated, selected, activated and engaged in antibody production. All these developmental and stimulatory processes are described in molecular and genetic terms to give a clear understanding of complex phenotyes. The molecular basis of many diseases due to B cell abnormality is also discussed. This definitive reference is directed at research level immunologists, molecular biologists and geneticists.

Molecular immunology. --- B cells. --- Immunoglobulins. --- Immunoglobulin genes. --- Antibody genes --- Ig genes --- Ir genes --- Antibodies --- Immune globulins --- Immune serum globulin --- Blood proteins --- Globulins --- Plasma cells --- Antibody diversity --- Antigens --- Bacterial immunoglobulin-binding proteins --- B lymphocytes --- Bone marrow derived cells --- Bursa equivalent cells --- Antigen presenting cells --- Lymphocytes --- Immune system --- Immunity --- Immunology --- Molecular biology --- Molecular aspects

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With 10 recent FDA approvals of therapeutic antibody drugs and hundreds more in the biotechnology pipeline, it is clear that engineered antibodies will play a major role in treating human diseases in the 21st century. In Antibody Engineering: Methods and Protocols, leading researchers and clinicians present a core collection of cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the generation of human recombinant antibodies by humanization approaches, molecular display technologies, and transgenic animals. The techniques for antibody expression and optimization utilize not only bacterial and mammalian cell cultures, but also insect cell cultures and transgenic plants. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics. Each fully tested protocol is described in step-by-step detail and includes background material, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. Comprehensive and up-to-date, Antibody Engineering: Methods and Protocols provides both laboratory and clinical investigators with a diverse array of the fundamental techniques upon which new and imaginative drug technologies can be successfully developed.

Immunotechnology --- Antibodies --- Protein Engineering --- therapeutic use --- genetics --- methods --- Electronic books. -- local. --- Immunology. --- Immunotechnology -- Laboratory manuals. --- Immunoglobulins --- Publication Formats --- Biology --- Investigative Techniques --- Genetic Engineering --- Biological Science Disciplines --- Genetic Techniques --- Immunoproteins --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Serum Globulins --- Publication Characteristics --- Natural Science Disciplines --- Blood Proteins --- Globulins --- Proteins --- Disciplines and Occupations --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Laboratory Manuals --- Genetics --- Methods --- Health & Biological Sciences --- Biomedical Engineering --- Therapeutic use --- Immunobiology --- Life sciences --- Serology --- Antibody engineering --- Immunological engineering --- Industrial immunology --- Biotechnology --- Immunology --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- Immunotechnology - Laboratory manuals --- Antibodies - therapeutic use - laboratory manuals --- Antibodies - genetics - laboratory manuals --- Protein Engineering - methods - laboratory manuals